Wright grew up in Bentonville, Arkansas, in what she referred to as "just a normal childhood." Wright attended the University of Arkansas, where she earned master's and law degrees, before entering the U.S. Army.

Wright earned a Master's Degree in National Security Affairs from the U.S. Naval War College in Newport, Rhode Island and later participated in reconstruction efforts after U.S. military actions in Grenada and Somalia.

Wright was stationed at Fort Bragg, North Carolina, from 1982 to 1984. One of her duties during that time was to draw up contingency plans for invading several countries, one of which was Iraq. She would later express dismay over what she considered the dismissal of such carefully laid plans in the actual invasion of Iraq in 2003.

Wright went on to serve 13 years in active duty in the U.S. Army, and 16 years in the Army Reserves, rising to the rank of colonel. She was placed in the Retired Ready Reserve, meaning the President could call her back to active duty in a time of need.

In 1987, Wright went to work for the Foreign Service within the U.S. State Department. Over the course of her State Department career, Wright served as Deputy Chief of Mission at the U.S. embassies in Afghanistan (which she helped open following the 2001 U.S. invasion of Afghanistan, an assignment she volunteered for), Sierra Leone (an embassy which she helped close and then reopen again), Micronesia and Mongolia, and also served at U.S. embassies in Uzbekistan (which she helped open), Kyrgyzstan, Grenada, and Nicaragua.

Wright's eventual resignation was not the first time she had spoken out against policy. In an interview, Wright said that she spoke out against United Nations bombing tactics waged in Somalia, in the effort to kill rebel leader Mohamed Farrah Aidid. Wright also says that she "held her nose" on multiple occasions, continuing her State Department work despite her own disagreements with the policy.

Wright submitted her resignation letter to then U.S. Secretary of State Colin Powell on March 19, 2003, the day before the onset of the U.S. invasion of Iraq. Her letter was published on the internet the following day.

In her resignation letter, Wright listed four reasons she could no longer work for the U.S. government under the Bush administration:

• The decision to invade Iraq without the blessing of the U.N. Security Council
• The "lack of effort" in the Israeli-Palestinian peace process
• The "lack of policy" in regard to North Korea
• The curtailment of civil liberties within the United States.

Wright was the third of three State Department officials to retire from service in protest in the month prior to the invasion of Iraq, the other two being Brady Kiesling and John H. Brown. Wright says that she did not know the other two, and had not read their resignation letters at the time she submitted her own.

Since her retirement from the State Department, Wright has become a prominent figure in the movement opposed to the occupation of Iraq. She has attended many conferences and given numerous lectures on her political views and on her experiences before and after her resignation.

Wright has worked with anti-war activist Cindy Sheehan on several occasions, most notably by helping organize the Camp Casey demonstration outside George W. Bush's Crawford, Texas, ranch in August 2005, and by accompanying the southern leg of the Bring Them Home Now bus tour. She also volunteered at Camp Casey 3, the Veterans For Peace shelter for Hurricane Katrina victims in Covington, Louisiana, during the bus tour.

Wright has willingly been arrested while taking part in anti-war demonstrations, the first such arrest occurring in front of the White House on September 26, 2005. She has said in interviews that she does not remove the arrest bracelets attached to her wrists upon the processing of her arrest, but rather collects them.

On October 19, 2005, Wright interrupted a Senate Foreign Relations Committee hearing, shouting at Secretary of State Condoleezza Rice, "Stop the war! Stop the killing!" Wright was uneventfully escorted out of the hearing room.

Wright served as one of five judges at the January 2006 sessions of the International Commission of Inquiry On Crimes Against Humanity Committed by the Bush Administration. She was also one of three recipients of the first annual Truthout Freedom and Democracy Awards.

Wright was one of three witnesses called to testify at an Article 32 hearing on behalf of U.S. Army Lt. Ehren Watada, who on June 22, 2006 refused to deploy to Iraq with his unit, asserting that the war violates both the United States Constitution and international law.

On April 1, 2007 Wright was cited, along with 38 other anti-nuclear activists, for trespassing at the Nevada Test Site during a Nevada Desert Experience event protesting against the continued development of nuclear weapons by the United States.That evening Wright appeared on The O'Reilly Factor to discuss the Geneva Conventions and how they applied to Iran in its taking of 15 British hostages. The discussion grew heated, and Wright stated that she had served 29 years in the military. During the course of the exchange, O'Reilly cut off her microphone.

On April 17, 2007, Wright attended a hearing of a House Foreign Affairs subcommittee. She was ejected from the hearing room after speaking out of turn in response to comments made by Republican Congressmember Dana Rohrabacher. On September 11, 2007, Wright was arrested, and later convicted, for disrupting a Senate Armed Services Committee hearing at which general David Petraeus and Ambassador to Iraq Ryan Crocker were testifying.[1]

Wright took part in a September 15, 2007 protest march and die-in on the steps of the United States Capitol Building, organized by the ANSWER Coalition and Iraq Veterans Against the War (IVAW). She was arrested for stepping over the wall after several IVAW and Veterans for Peace members were arrested. Here is a link to YouTube video of those arrests that clearly show Ann Wright being arrested by Capitol Police: http://www.youtube.com/watch?v=Pz9ekYNjaKQ

On October 3, 2007, Wright and Code Pink activist Medea Benjamin were denied entry to Canada because their names appear on an FBI watchlist due to arrests related to their anti-war activism.

In December 2008, Wright has recently expressed her dissatisfaction with the current U.S foreign policy toward Palestine .

In 2009 Wright began work as a leading member of the steering committee for the Gaza Freedom March.

On June 1, 2010 Ann Wright was seen being escorted by Israeli Defense Forces from the attempt to break the blockade of the Gaza strip.

On June 3, 2010 Wright was interviewed by Democracy Now! She was on the Challenger 1, and observed the Israeli soldiers rappeling down from helicopters onto the deck of the Turkish ship Marmara. Her own ship was boarded. "Flash bangs were used. One of our journalists was hit with something of an electric shock. I don’t know that it was a taser."

While on tour afterwards, she claimed that she witnessed the "murder [of] nine innocent civilians". However, in an interview with Aaron Lerner of IMRA, she admitted that she did not actually see the clash between the IDF soldiers and the armed passengers on board the Mavi Marmara. In fact, it was dark outside because it was pre-dawn, and she was admittedly on another ship, the Challenger, which was about 150 yards away from the Marmara.

In 2008, Koa Books published Dissent, Voices of Conscience, co-authored by Ann Wright and Susan Dixon. The book is subtitled, Government Insiders Speak Out Against the War in Iraq. Longtime anti-war activist Daniel Ellsberg, who leaked the top-secret Pentagon Papers in 1971, wrote the foreword to the book, which says, "This is a book that should be leaked into the government."